9/19/2023 0 Comments Facs flowjo graphIn the example below a Query gate was once again overlaid with the parent population, but now that population is displayed in a series of 2D plots, allowing a cytometrist to view a complex population identified in this case on a tSNE map in a traditional manner.Ĭolor map plots display the original graph heat mapped for as many parameters as selected. NxN Plots display every combination of parameters in the population. In the example below a Query gate is again overlaid with the parent population and the all-by-Y plot is used to highlight the expression of CCR9 in conjunction with each of the other markers for both populations. In this example we can see the highlighted population is expressing more CD20, HLA-DR, and CD16 than most of the other cells.Īll Parameters by Y will provide 2D plots with every parameter versus the current Y axis of the primary plot. Including the parent population in the overlay provides context to interpret the histograms on the Query gate. In the example below a Query gate is overlaid on the parent population, with multigraph histograms enabled, including seven parameters. Right-clicking on the multi-histogram plot will bring up a dialog for showing or hiding parameters. If you have multiple populations overlaid, each of those populations will be in each histogram with the color preserved from the original plot. Histograms will provide you a multigraph with a histogram of every parameter in the data file. You’ll see a fourth option, Multigraph Color Mapping, if you have right-clicked on a single population plot, as that option is only possible on single populations: If you expand the Multigraph interface, you’ll notice three options. All options work with single samples, but most work with multiple samples/populations overlaid, creating graphics that emphasize comparison. At the bottom of the right-click interface, there is an option for Multigraph called “Make Multigraph Overlay”. To access the Multigraph interface in version 10, place a plot in the layout editor and right click on the plot. The Multigraph feature is a tool for exploring data in n-dimensions using a variety of graphical options.
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